Applications

Cultivation and high-resolution imaging of cells

Immunofluorescence staining

Widefield and confocal fluorescence microscopy of living and fixed cells

Live cell imaging over extended time periods

Transfection assays

Differential interference contrast (DIC) when using a DIC Lid

Cell location and counting when using the gridded version

Specifications

Technical Features

Standard format imaging dish with a 35 mm diameter for tissue culture

ibiTreat (tissue culture-treated) surface for optimal cell adhesion and cell growth

High walls with a standard height for easy handling; low wall version available: μ-Dish 35 mm, low

Lid with locking feature for minimal evaporation

Rim for easy opening

No autofluorescence

Biocompatible plastic material—no glue, no leaking

Available as a Bulk Pack with 400 individually packed μ-Dishes per box

Available with a #1.5H Glass Coverslip Bottom: μ-Dish 35 mm, high Glass Bottom, specifically suitable for TIRF and single molecule applications

Available with a 500 μm grid for cell counting: μ-Dish 35 mm, high Grid-500

Available with a non-adhesive Bioinert surface: μ-Dish 35 mm, high Bioinert, specifically suitable for 3D applications, such as spheroids and suspension cells

The Principle of the μ-Dish 35 mm, high

The Coverslip Bottom

The μ-Dish 35 mm, high comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available with a #1.5H Glass Coverslip Bottom for TIRF or single molecule microscopy.

As another option for cost-effective high-throughput experiments, we offer the Glass Bottom Dish 35 mm with a #1.5 glass coverslip bottom.

The ibiTreat Surface

ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.

Lid with Locking Feature for Minimized Evaporation

All ibidi μ-Dishes are equipped with the special lid-locking feature. The locking position minimizes evaporation, and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

Experimental Examples：

ibidi μ-Dish Height Variations

μ-Dish 35 mm, high

High walls for all standard cell culture applications

μ-Dish 35 mm, low

Low walls that provide greater cell access, which is useful for micromanipulation

Triple immunofluorescence of bovine endothelial cells.

Red: mitochondria, stained with MitoTracker? Red CMXRos;

Green: F-actin, stained with Alexa Fluor? 488 Phalloidin;

Blue: nuclei, stained with DAPI.

Comparison of the ibidi Dishes 35 mm

Please find a detailed comparison of the material specifications, including suitable microscopy applications for the ibidi Polymer Coverslip, the ibidi Glass Bottom, and further materials here.